Publicaciones
Dra. Ana Pardo-Saganta
Bárcena-Varela M., Paish HL., Álvarez L., Uriarte I., Latasa MU., Santamaría E., Recalde M., Gárate M., Clavería A., Colyn L., Arechederra M., Iraburu MJ., Milkiewitz M., Milkiewitz P., Sangro B., Robinson SM., French J., Pardo-Saganta A., Oyarzábal J., Prósper F., Rombouts K., Oakley F., Mann J., Berasain C., Avila MA., Fernández-Barrena MG. Epigenetic mechanisms and metabolic reprogramming in fibrogenesis: Dual targeting of G9a and DNMT1 for the inhibition of liver fibrosis. Gut, March 2020, gutjnl-2019-320205. doi: 10.1136/gutjnl-2019-320205.
Pardo-Saganta A., Calvo, I.A., Saez, B. & Prosper F. Role of the extracellular matrix in stem cell maintenance. Curr Stem Cell Rep March 2019, 5:1-10. doi:10.1007/s40778-019-0149-9.
Causton B, Pardo-Saganta A, Gillis J, Discipio K, Kooistra T, Rajagopal J, Xavier RJ, Cho JL, Medoff BD. CARMA3 Mediates Allergic Lung Inflammation in Response to Alternaria alternata. Am J Respir Cell Mol Biol. 2018, 59(6):684-694. doi: 10.1165/rcmb.2017-0181OC.
Pardo-Saganta A, Tata PR, Law BM, Saez B, Chow RDz, Prabhu M., Gridley, T, Rajagopal J. Parent stem cells can serve as niches for their daughter cells. Nature. 2015 Jul 30;523(7562):597-601. doi: 10.1038/nature14553.
Ren B, Azzegagh Z, Jaramillo AM, Zhu Y, Pardo-Saganta A, Bagirzadeh R, Flores JR, Han W, Tang YJ, Tu J, Alanis DM, Evans CM, Guindani M, Roche PA, Rajagopal J, Chen J, Davis CW, Tuvim MJ, Dickey BF. SNAP23 is selectively expressed in airway cells and mediates baseline and stimulated mucin secretion. Biosci Rep. 2015 Apr 28;35(3). doi: 10.1042/BSR20150004.
Causton B, Ramadas RA, Cho JL, Jones K, Pardo-Saganta A, Rajagopal J, Xavier RJ, Medoff BD. CARMA3 is critical for the initiation of allergic airway inflammation. J Immunol. 2015 Jul 15;195(2):683-94. doi: 10.4049/jimmunol.1402983.
Pardo-Saganta A, Law B, Tata PR, Villoria J, Saez B, Mou H, Zhao R, Rajagopal J. Injury induces direct lineage segregation of functionally distinct airway basal stem/progenitor cell subpopulations. Cell Stem Cell. 2015 Feb 5;16(2):184-97. doi: 10.1016/j.stem.2015.01.002.
Yu VWC, Saez B, Cook C, Lotinun S, Pardo-Saganta A, Wang YH, Lymperi S, Raaijmakers MH, Wu JY, Zhou L, Rajagopal J, Kronenberg HM, Baron R, Scadden DT. Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow. J Exp Med. 2015 May 4;212(5):759-74. doi: 10.1084/jem.20141843.
Saez B, Ferraro F, Yusuf RZ, Cook CM, Yu VW, Pardo-Saganta A, Sykes SM, Palchaudhuri R, Schajnovitz A, Lotinun S, Lymperi S, Mendez-Ferrer S, Del Toro R, Day R, Vasic R, Acharya SS, Baron R, Lin CP, Yamaguchi Y, Wagers AJ, Scadden DT. Inhibiting stromal cell heparan sulfate synthesis improves stem cell mobilization and enables engraftment without cytotoxic conditioning. Blood 2014, 124(19): 2937-2947. doi: 10.1182/blood-2014-08-593426.
Zhao R, Fallon TR, Saladi SV, Pardo-Saganta A, Villoria J, Mou H, Vinarsky V, Gonzalez-Celeiro M, Nunna N, Hariri LP, Camargo F, Ellisen LW, Rajagopal J. Yap tunes airway epithelial size and architecture by regulating the identity, maintenance, and self-renewal of stem cells. Dev Cell. 2014 30 (2):151-165. doi: 10.1016/j.devcel.2014.06.004.
Tata PR, Mou H, Pardo-Saganta A, Zhao R, Prabhu M, Law BM, Vinarsky V, Cho JL, Breton S, Sahay A, Medoff BD, Rajagopal J. Dedifferentiation of committed epithelial cells into stem cells in vivo. Nature. 2013 Nov 14;503 (7475):218-23. doi: 10.1038/nature12777.
Tata PR, Pardo-Saganta A, Prabhu M, Vinarsky V, Law BM, Fontaine BA, Tager AM, Rajagopal J. Airway specific inducible transgene expression using aerosolized doxycycline. Am J Respir Cell Mol Biol. 2013 Dec;49(6):1048-56. doi: 10.1165/rcmb.2012-0412OC.
Santamaria M, Pardo-Saganta A, Alvarez-Asiain L, Di Scala M, Qian C, Prieto J, Avila MA. Nuclear α1-antichymotrypsin promotes chromatin condensation and inhibits proliferation of human hepatocellular carcinoma cells. Gastroenterology. 2013 Apr;144(4):818-828.e4. doi: 10.1053/j.gastro.2012.12.029.
Pardo-Saganta A, Law BM, Gonzalez-Celeiro M, Vinarsky V, Rajagopal J. Ciliated cells of pseudostratified airway epithelium do not become mucous cells after OVA challenge. Am J Respir Cell Mol Biol. 2013 Mar;48(3):364-73. doi: 10.1165/rcmb.2012-0146OC.
Kim JK, Vinarsky V, Wain JC, Zhao R, Jung K, Choi J, Lam A, Pardo-Saganta A, Breton S, Rajagopal J, Yun SH. In vivo imaging of tracheal epithelial cells in mice during airway regeneration. Am J Respir Cell Mol Biol. 2012 Dec;47(6):864-8. doi: 10.1165/rcmb.2012-0164OC.
Pardo-Saganta A, Latasa MU, Castillo J, Alvarez L, Perugorria MJ, Sarobe P, Rodriguez-Ortigosa CM, Prieto J, Berasain C, Santamaría M, Avila MA. (2009) The EGFR ligand amphiregulin is a negative regulator of hepatic acute phase gene expression. Journal of Hepatology 51(6):1010-20, 2009. doi: 10.1016/j.jhep.2009.06.030.
Lorenzo-Zuniga V, Rodriguez-Ortigosa CM, Bartoli R, Martinez-Chantar ML, Martinez-Peralta L, Pardo A, Ojanguren I, Quiroga J, Planas R, Prieto J. Insulin-like growth factor I improves intestinal barrier function in cirrhotic rats. Gut 55(9):1306-12, 2006. doi: 10.1136/gut.2005.079988.
Sanz S, Pucilowska JB, Liu S, Rodríguez-Ortigosa CM, Lund PK, Brenner DA, Fuller CR, Simmons JG, Pardo A, Martinez-Chantar ML, Fagin JA, Prieto J. Expression of insulin-like growth factor I by activated hepatic stellate cells reduces fibrogenesis and enhances regeneration after liver injury. Gut 54(1):134-41, 2005. doi: 10.1136/gut.2003.024505.
Dr. Borja Saez
Yusuf R, Saez B, Sharda A, van Gastel N, Yu V, Baryawno N, Scadden EW, Acharya SS, Chattophadhyay S, Huang C, Viswanathan V, S’Aulis D, Cobert J, Sykes DB, Keibler MA, Das S, Hutchinson JN, Churchill M, Mukherjee S, Lee D, Mercier FE, Doench J, Bullinger L, Logan DJ, Schreiber S, Stephanopoulos G, Rizzo WB, Scadden DT. Aldehyde dehydrogenase 3a2 protects AML cells from oxidative death and the synthetic lethality of ferroptosis inducers. Blood. 2020. doi: 10.1182/blood.2019001808.
Alameda D, Saez B, Lara-Astiaso D, Sarvide S, Lasa M, Alignani D, Rodriguez I, Garate S, Vilas A, Paiva B, Alfonso-Olmos M, Lamo de Espinosa JM, Prosper F, San Miguel JF, Maiso P. Characterization of freshly isolated mesenchymal stromal cells from healthy and multiple myeloma bone marrow: transcriptional modulation of the microenvironment. Haematologica. 2020. doi: 10.3324/haematol.2019.235135.
Czechowicz A, Palchaudhuri R, Scheck A, Hu Y, Hoggatt J, Saez B, Pang WW, Mansour MK, Tate TA, Chan YY, Walck E, Wernig G, Shizuru JA, Winau F, Scadden DT, Rossi DJ. Selective hematopoietic stem cell ablation using CD117-antibody-drug-conjugates enables safe and effective transplantation with immunity preservation. Nature Communications. 2019;10(1):617. doi: 10.1038/s41467-018-08201-x.
Shirai CL, White BS, Tripathi M, Tapia R, Ley JN, Ndonwi M, Kim S, Shao J, Carver A, Saez B, Fulton RS, Fronick C, O’Laughlin M, Lagisetti C, Webb TR, Graubert TA, Walter MJ. Mutant U2AF1-expressing cells are sensitive to pharmacological modulation of the spliceosome. Nature Communications. 2017;8:14060. doi: 10.1038/ncomms14060.
Saez B, Walter MJ, Graubert TA. Splicing factor gene mutations in hematologic malignancies. Blood. 2017;129(10):1260-1269. doi: 10.1182/blood-2016-10-692400.
Nguyen HD, Yadav T, Giri S, Saez B, Graubert TA, Zou L. Functions of Replication Protein A as a Sensor of R Loops and a Regulator of RNaseH1. Molecular Cell. 2017;65(5):832-847 e834. doi: 10.1016/j.molcel.2017.01.029.
Yu VWC, Yusuf RZ, Oki T, Wu J, Saez B, Wang X, Cook C, Baryawno N, Ziller MJ, Lee E, Gu H, Meissner A, Lin CP, Kharchenko PV, Scadden DT. Epigenetic Memory Underlies Cell-Autonomous Heterogeneous Behavior of Hematopoietic Stem Cells. Cell. 2016;167(5):1310-1322 e1317. doi: 10.1016/j.cell.2016.10.045.
Ramasamy S, Saez B, Mukhopadhyay S, Ding D, Ahmed AM, Chen X, Pucci F, Yamin R, Wang J, Pittet MJ, Kelleher CM, Scadden DT, Sweetser DA. Tle1 tumor suppressor negatively regulates inflammation in vivo and modulates NF-kappaB inflammatory pathway. Proc Natl Acad Sci U S A. 2016;113(7):1871-1876. doi: 10.1073/pnas.1511380113.
Palchaudhuri R, Saez B, Hoggatt J, Schajnovitz A, Sykes DB, Tate TA, Czechowicz A, Kfoury Y, Ruchika F, Rossi DJ, Verdine GL, Mansour MK, Scadden DT. Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin. Nature Biotechnology. 2016;34(7):738-745. doi: 10.1038/nbt.3584.
Yu VW, Saez B, Cook C, Lotinun S, Pardo-Saganta A, Wang YH, Lymperi S, Ferraro F, Raaijmakers MH, Wu JY, Zhou L, Rajagopal J, Kronenberg HM, Baron R, Scadden DT. Specific bone cells produce DLL4 to generate thymus-seeding progenitors from bone marrow. J Exp Med. 2015;212(5):759-774. doi: 10.1084/jem.20141843.
Pardo-Saganta A, Tata PR, Law BM, Saez B, Chow RD, Prabhu M, Gridley T, Rajagopal J. Parent stem cells can serve as niches for their daughter cells. Nature. 2015;523(7562):597-601. doi: 10.1038/nature14553.
Pardo-Saganta A, Law BM, Tata PR, Villoria J, Saez B, Mou H, Zhao R, Rajagopal J. Injury induces direct lineage segregation of functionally distinct airway basal stem/progenitor cell subpopulations. Cell Stem Cell. 2015;16(2):184-197. doi: 10.1016/j.stem.2015.01.002.
Dutta P, Sager HB, Stengel KR, Naxerova K, Courties G, Saez B, Silberstein L, Heidt T, Sebas M, Sun Y, Wojtkiewicz G, Feruglio PF, King K, Baker JN, van der Laan AM, Borodovsky A, Fitzgerald K, Hulsmans M, Hoyer F, Iwamoto Y, Vinegoni C, Brown D, Di Carli M, Libby P, Hiebert SW, Scadden DT, Swirski FK, Weissleder R, Nahrendorf M. Myocardial Infarction Activates CCR2(+) Hematopoietic Stem and Progenitor Cells. Cell Stem Cell. 2015;16(5):477-487. doi: 10.1016/j.stem.2015.04.008.
Saez B, Ferraro F, Yusuf RZ, Cook CM, Yu VW, Pardo-Saganta A, Sykes SM, Palchaudhuri R, Schajnovitz A, Lotinun S, Lymperi S, Mendez-Ferrer S, Toro RD, Day R, Vasic R, Acharya SS, Baron R, Lin CP, Yamaguchi Y, Wagers AJ, Scadden DT. Inhibiting stromal cell heparan sulfate synthesis improves stem cell mobilization and enables engraftment without cytotoxic conditioning. Blood. 2014;124(19):2937-2947. doi: 10.1182/blood-2014-08-593426.
Choi YJ, Saez B, Anders L, Hydbring P, Stefano J, Bacon NA, Cook C, Kalaszczynska I, Signoretti S, Young RA, Scadden DT, Sicinski P. D-cyclins repress apoptosis in hematopoietic cells by controlling death receptor Fas and its ligand FasL. Developmental Cell. 2014;30(3):255-267. doi: 10.1016/j.devcel.2014.06.015.
Zhang H, Alberich-Jorda M, Amabile G, Yang H, Staber PB, Di Ruscio A, Welner RS, Ebralidze A, Zhang J, Levantini E, Lefebvre V, Valk PJ, Delwel R, Hoogenkamp M, Nerlov C, Cammenga J, Saez B, Scadden DT, Bonifer C, Ye M, Tenen DG. Sox4 is a key oncogenic target in C/EBPalpha mutant acute myeloid leukemia. Cancer Cell. 2013;24(5):575-588. doi: 10.1016/j.ccr.2013.09.018.
Simic P, Zainabadi K, Bell E, Sykes DB, Saez B, Lotinun S, Baron R, Scadden D, Schipani E, Guarente L. SIRT1 regulates differentiation of mesenchymal stem cells by deacetylating beta-catenin. EMBO Mol Med. 2013;5(3):430-440. doi: 10.1002/emmm.201201606.
Sykes SM, Lane SW, Bullinger L, Kalaitzidis D, Yusuf R, Saez B, Ferraro F, Mercier F, Singh H, Brumme KM, Acharya SS, Scholl C, Tothova Z, Attar EC, Frohling S, DePinho RA, Armstrong SA, Gilliland DG, Scadden DT. AKT/FOXO signaling enforces reversible differentiation blockade in myeloid leukemias. Cell. 2011;146(5):697-708. doi: 10.1016/j.cell.2011.07.032.
Steinbicker AU, Sachidanandan C, Vonner AJ, Yusuf RZ, Deng DY, Lai CS, Rauwerdink KM, Winn JC, Saez B, Cook CM, Szekely BA, Roy CN, Seehra JS, Cuny GD, Scadden DT, Peterson RT, Bloch KD, Yu PB. Inhibition of bone morphogenetic protein signaling attenuates anemia associated with inflammation. Blood. 2011;117(18):4915-4923. doi: 10.1182/blood-2010-10-313064.
Ferraro F, Lymperi S, Mendez-Ferrer S, Saez B, Spencer JA, Yeap BY, Masselli E, Graiani G, Prezioso L, Rizzini EL, Mangoni M, Rizzoli V, Sykes SM, Lin CP, Frenette PS, Quaini F, Scadden DT. Diabetes impairs hematopoietic stem cell mobilization by altering niche function. Science Translational Medicine. 2011;3(104):104ra101. doi: 10.1126/scitranslmed.3002191.
Gurumurthy S, Xie SZ, Alagesan B, Kim J, Yusuf RZ, Saez B, Tzatsos A, Ozsolak F, Milos P, Ferrari F, Park PJ, Shirihai OS, Scadden DT, Bardeesy N. The Lkb1 metabolic sensor maintains haematopoietic stem cell survival. Nature. 2010;468(7324):659-663. doi: 10.1038/nature09572.
Agirre X, Jimenez-Velasco A, San Jose-Eneriz E, Garate L, Bandres E, Cordeu L, Aparicio O, Saez B, Navarro G, Vilas-Zornoza A, Perez-Roger I, Garcia-Foncillas J, Torres A, Heiniger A, Calasanz MJ, Fortes P, Roman-Gomez J, Prosper F. Down-regulation of hsa-miR-10a in chronic myeloid leukemia CD34+ cells increases USF2-mediated cell growth. Mol Cancer Res. 2008;6(12):1830-1840. doi: 10.1158/1541-7786.MCR-08-0167.
Saez B, Martin-Subero JI, Odero MD, Prosper F, Cigudosa JC, Schoch R, Calasanz MJ, Siebert R. Multicolor interphase cytogenetics for the study of plasma cell dyscrasias. Oncol Rep. 2007;18(5):1099-1106.
Saez B, Martin-Subero JI, Lahortiga I, Largo C, Larrayoz MJ, Odero MD, Prosper F, Cigudosa JC, Siebert R, Calasanz MJ. Simultaneous translocations of FGFR3/MMSET and CCND1 into two different IGH alleles in multiple myeloma: lack of concurrent activation of both proto-oncogenes. Cancer Genet Cytogenet. 2007;175(1):65-68. doi: 10.1016/j.cancergencyto.2006.12.009.
Largo C, Saez B, Alvarez S, Suela J, Ferreira B, Blesa D, Prosper F, Calasanz MJ, Cigudosa JC. Multiple myeloma primary cells show a highly rearranged unbalanced genome with amplifications and homozygous deletions irrespective of the presence of immunoglobulin-related chromosome translocations. Haematologica. 2007;92(6):795-802. doi: 10.3324/haematol.11052.
Saez B, Martin-Subero JI, Odero MD, Prosper F, Hernandez R, Cigudosa JC, Siebert R, Calasanz MJ. Interphase FISH for the detection of breakpoints in IG loci and chromosomal changes with adverse prognostic impact in multiple myeloma with normal karyotypes. Cancer Genet Cytogenet. 2006;167(2):183-185. doi: 10.1016/j.cancergencyto.2005.12.008.
Saez B, Martin-Subero JI, Largo C, Martin MC, Odero MD, Prosper F, Siebert R, Calasanz MJ, Cigudosa JC. Identification of recurrent chromosomal breakpoints in multiple myeloma with complex karyotypes by combined G-banding, spectral karyotyping, and fluorescence in situ hybridization analyses. Cancer Genet Cytogenet. 2006;169(2):143-149. doi: 10.1016/j.cancergencyto.2006.04.008.
Largo C, Alvarez S, Saez B, Blesa D, Martin-Subero JI, Gonzalez-Garcia I, Brieva JA, Dopazo J, Siebert R, Calasanz MJ, Cigudosa JC. Identification of overexpressed genes in frequently gained/amplified chromosome regions in multiple myeloma. Haematologica. 2006;91(2):184-191.
Martin-Subero JI, Odero MD, Hernandez R, Cigudosa JC, Agirre X, Saez B, Sanz-Garcia E, Ardanaz MT, Novo FJ, Gascoyne RD, Calasanz MJ, Siebert R. Amplification of IGH/MYC fusion in clinically aggressive IGH/BCL2-positive germinal center B-cell lymphomas. Genes Chromosomes Cancer. 2005;43(4):414-423. doi: 10.1002/gcc.20187.
Lahortiga I, Belloni E, Vazquez I, Agirre X, Larrayoz MJ, Vizmanos JL, Valganon M, Zudaire I, Saez B, Mateos MC, Di Fiore PP, Calasanz MJ, Odero MD. NUP98 is fused to HOXA9 in a variant complex t(7;11;13;17) in a patient with AML-M2. Cancer Genet Cytogenet. 2005;157(2):151-156. doi: 10.1016/j.cancergencyto.2004.08.001.
Saez B, Martin-Subero JI, Guillen-Grima F, Odero MD, Prosper F, Cigudosa JC, Harder L, Calasanz MJ, Siebert R. Chromosomal abnormalities clustering in multiple myeloma reveals cytogenetic subgroups with nonrandom acquisition of chromosomal changes. Leukemia. 2004;18(3):654-657. doi: 10.1038/sj.leu.2403256.